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DGCR8 antibody

Cat.#: 107241

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Product Information

  • Product Name
    DGCR8 antibody
  • Documents
  • Description
    DGCR8 Mouse Monoclonal antibody. Positive WB detected in K562 cells, BxPC-3 cells, HepG2 cells, human brain tissue, K-562 cells. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 120 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    C22orf12 antibody; DGCR8 antibody; DGCRK6 antibody; Gy1 antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of DGCR8 recombinant protein (Accession Number: BC009323). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107241(DGCR8 antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107241(DGCR8 antibody) at dilution of 1:1000

    IP Result of anti-DGCR8 (IP:Catalog No:107241, 5ug; Detection:Catalog No:107241 1:500) with K-562 cells lysate 3440ug.

    IP Result of anti-DGCR8 (IP:Catalog No:107241, 5ug; Detection:Catalog No:107241 1:500) with K-562 cells lysate 3440ug.

  • Background
    DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is a 82-86 kDa protein, but the post-modified DGCR8 is about 120 kDa.
  • References
    • Peric D, Chvalova K, Rousselet G. Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs. Oncogene. 31(16):2039-48. 2012.
    • Lugli G, Larson J, Demars MP, Smalheiser NR. Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain. Journal of neurochemistry. 123(4):459-66. 2012.
    • Zhu C, Chen C, Huang J. SUMOylation at K707 of DGCR8 controls direct function of primary microRNA. Nucleic acids research. 43(16):7945-60. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"