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Product Name
DFNA5 Polyclonal Antibody
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Description
Polyclonal antibody to DFNA5
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DFNA5 antibody; ICERE-1 antibody; non-syndromic hearing impairment protein 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DFNA5 (NP_004394.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - DFNA5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DFNA5 Antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunohistochemistry - DFNA5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using DFNA5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DFNA5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using DFNA5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DFNA5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using DFNA5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DFNA5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using DFNA5 antibody at dilution of 1:100 (40x lens).
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Background
Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53.; Gasdermin-E, N-terminal: Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents. Exhibits bactericidal activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"