Dermaseptin-S4 peptide

Dermaseptin-S4 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

ALWMTLLKKVLKAAAKAALNAVLVGANA

H-Ala-Leu-Trp-Met-Thr-Leu-Leu-Lys-Lys-Val-Leu-Lys-Ala-Ala-Ala-Lys-Ala-Ala-Leu-Asn-Ala-Val-Leu-Val-Gly-Ala-Asn-Ala-OH

28

95.02%

C₁₃₂H₂₂₉N₃₅O₃₂S

2850.4

151896-15-0

16130878

Synthetic

Powder

It showed activities against P. falciparum (antimalarial) as well as human sperm. Based on the primary structure, it can be fitted to a class L amphipathic alpha helix which places all K on the polar face. The N-terminal region is responsible for both oligomerization and hemolytic activity. NMR structures of two derivatives have been solved [2DD6 for K4-S4(1-13) and 2DCX for NC12-K4S4(1-13)]. Sequence has been corrected based on the original paper. 3/2015 GW. Dermaseptin-S4 was found in Sauvage's leaf frog, Phyllomedusa sauvagii, South America.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mor A, Nicolas P. Isolation and structure of novel defensive peptides from frog skin. Eur J Biochem. 1994;219(1-2):145-54.
• Krugliak M, Feder R, Zolotarev VY, Gaidukov L, Dagan A, Ginsburg H, Mor A. Antimalarial activities of dermaseptin S4 derivatives. Antimicrob Agents Chemother. 2000 Sep;44(9):2442-51.
• Sztukowska MN, Roky M, Demuth DR. Peptide and non-peptide mimetics as potential therapeutics targeting oral bacteria and oral biofilms. Mol Oral Microbiol. 2019 Oct;34(5):169-182.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.