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Product Name
DENND1A antibody
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Description
DENND1A Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in A431 cells. Positive IP detected in K-562 cells. Positive WB detected in SKOV-3 cells, K-562 cells. Observed molecular weight by Western-blot: 111 kDa, 64 kDa
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Tested applications
ELISA, IF, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Connecdenn antibody; DENN/MADD domain containing 1A antibody; DENND1A antibody; FAM31A antibody; FLJ38464 antibody; KIAA1608 antibody; Protein FAM31A antibody; RP11 230L22.3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DENND1A recombinant protein (Accession Number: NM_024820). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109888(DENND1A Antibody) at dilution of 1:600
IP Result of anti-DENND1A (IP:Catalog No:109888, 4ug; Detection:Catalog No:109888 1:600) with K-562 cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109888(DENND1A Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109888(DENND1A Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of A431 cells using Catalog No:109888(DENND1A Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DENND1A (DENN/MADD domain containing 1A), also known as Connecdenn,FAM31A or KIAA1608, is a 1,009 amino acid peripheral membrane protein. Recent studies has found DENND1A is strongly associated with PCOS (Polycystic ovary syndrome). DENND1A has several isoforms.This antibody(25658-1-AP) can recognize all the isoforms at 112 kDa, 52-64 kDa.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"