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Product Name
DEK antibody
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Description
DEK Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A375 cells, A431 cells, K-562 cells, mouse brain tissue, rat brain tissue. Positive IHC detected in human brain tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 43-50kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
D6S231E antibody; DEK antibody; DEK oncogene antibody; Protein DEK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DEK recombinant protein (Accession Number: XM_024446544). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109886(DEK antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human brain using Catalog No:109886(DEK antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109886(DEK antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using DEK antibody Catalog No:109886 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DEK (IP:Catalog No:109886, 4ug; Detection:Catalog No:109886 1:500) with HeLa cells lysate 1200ug.
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Background
Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptinoal regulaton and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.
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References
- Wang J, Sun L, Yang M. DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 61(7):510-21. 2013.
- Begum NA, Stanlie A, Nakata M, Akiyama H, Honjo T. The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation. The Journal of biological chemistry. 287(39):32415-29. 2012.
- Lin CJ, Koh FM, Wong P, Conti M, Ramalho-Santos M. Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote. Developmental cell. 30(3):268-79. 2014.
- Matrka MC, Hennigan RF, Kappes F. DEK over-expression promotes mitotic defects and micronucleus formation. Cell cycle (Georgetown, Tex.). 14(24):3939-53. 2015.
- Cheung TH, Quach NL, Charville GW. Maintenance of muscle stem-cell quiescence by microRNA-489. Nature. 482(7386):524-8. 2012.
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