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DDX6 antibody

Cat.#: 109842

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Product Information

  • Product Name
    DDX6 antibody
  • Documents
  • Description
    DDX6 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HeLa cells, mouse testis tissue. Positive IP detected in mouse testis tissue. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 54kd
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ATP dependent RNA helicase p54 antibody; DDX6 antibody; DEAD box protein 6 antibody; FLJ36338 antibody; HLR2 antibody; Oncogene RCK antibody; P54 antibody; RCK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DDX6 recombinant protein (Accession Number: NM_004397). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109842(P54 antibody) at dilution of 1:500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109842(P54 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109842(P54 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109842(P54 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109842(P54 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109842(P54 antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-P54 (IP:Catalog No:109842, 4ug; Detection:Catalog No:109842 1:500) with mouse testis tissue lysate 4000ug.

    IP Result of anti-P54 (IP:Catalog No:109842, 4ug; Detection:Catalog No:109842 1:500) with mouse testis tissue lysate 4000ug.

  • Background
    P54, also named as DDX6, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.
  • References
    • Chappell VA, Busada JT, Keiper BD, Geyer CB. Translational activation of developmental messenger RNAs during neonatal mouse testis development. Biology of reproduction. 89(3):61. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"