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Product Name
DDX58 antibody
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Description
DDX58 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, MCF7 cells, mouse brain tissue. Positive IP detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 95 kDa,100 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DDX58 antibody; DEAD box protein 58 antibody; DKFZp434J1111 antibody; DKFZp686N19181 antibody; FLJ13599 antibody; RIG 1 antibody; RIG I antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_014314). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109840(DDX58 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using DDX58 antibody Catalog No:109840 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-DDX58 (IP:Catalog No:109840, 4ug; Detection:Catalog No:109840 1:300) with HepG2 cells lysate 3600ug.
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Background
DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.
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References
- Zhang W, Zhang L, Zan Y, Du N, Yang Y, Tien P. Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins. The Journal of general virology. 96(Pt 1):170-82. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"