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Product Name
DDX47 Polyclonal Antibody
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Description
Polyclonal antibody to DDX47
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
DDX47 antibody; E4-DBP antibody; HQ0256 antibody; MSTP162 antibody; RRP3 antibody; DEAD-box helicase 47 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human DDX47 (NP_057439.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - DDX47 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DDX47 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Involved in apoptosis. May have a role in rRNA processing and mRNA splicing. Associates with pre-rRNA precursors.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"