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Product Name
DDX41 Polyclonal Antibody
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Description
Polyclonal antibody to DDX41
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
DDX41 antibody; ABS antibody; MPLPF antibody; DEAD-box helicase 41 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200 -
Validations
Western blot - DDX41 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DDX41 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - DDX41 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX41 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX41 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - DDX41 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using DDX41 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - DDX41 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DDX41 antibody . Western blot was performed from the immunoprecipitate using DDX41 antibody at a dilition of 1:1000.
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Background
Probable ATP-dependent RNA helicase. Is required during post-transcriptional gene expression. May be involved in pre-mRNA splicing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"