-
Product Name
DDX4,VASA antibody
- Documents
-
Description
DDX4,VASA Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 79 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
DDX4 antibody; DEAD box protein 4 antibody; VASA antibody; Vasa homolog antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of DDX4,VASA recombinant protein (Accession Number: NM_001142549). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
-
Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:109830(DDX4,VASA antibody) at dilution of 1:200
IP Result of anti-DDX4,VASA (IP:Catalog No:109830, 4ug; Detection:Catalog No:109830 1:300) with mouse testis tissue lysate 4000ug.
-
Background
DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"