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Product Name
DDX3 antibody
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Description
DDX3 Rabbit Polyclonal antibody. Positive IF detected in Ethacrynic acid treated HepG2 cells, HepG2 cells. Positive IHC detected in human cervical cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in mouse liver tissue, C6 cells, HeLa cells, HepG2 cells, HT-1080 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 73 kDa, 60-65 kDa
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DBX antibody; DDX14 antibody; DDX3 antibody; DDX3X antibody; DEAD box antibody; X isoform antibody; Helicase like protein 2 antibody; HLP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDX3 recombinant protein (Accession Number: NM_001356). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109827(DDX3 Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109827(DDX3 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-DDX3 (IP:Catalog No:109827, 4ug; Detection:Catalog No:109827 1:300) with HeLa cells lysate 2480ug.
Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using Catalog No:109827(DDX3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.
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