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Product Name
DDX20 Polyclonal Antibody
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Description
Polyclonal antibody to DDX20
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DDX20 antibody; DP103 antibody; GEMIN3 antibody; DEAD-box helicase 20 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human DDX20 (NP_009135.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - DDX20 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DDX20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - DDX20 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using DDX20 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX20 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human amygdalitis using DDX20 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX20 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using DDX20 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - DDX20 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using DDX20 antibody . Blue: DAPI for nuclear staining.
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Background
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"