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Product Name
DDX17 antibody
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Description
DDX17 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HEK-293 cells. Positive IF detected in Hela cells. Positive IHC detected in human pancreas tissue, human kidney tissue. Observed molecular weight by Western-blot: 72 kDa,80 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DDX17 antibody; DEAD box protein 17 antibody; DEAD box protein p72 antibody; DKFZp761H2016 antibody; P72 antibody; RH70 antibody; RNA dependent helicase p72 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDX17 recombinant protein (Accession Number: NM_006386). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109781(DDX17,P72 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:109781(DDX17,P72 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109781(DDX17,P72 antibody) at dilution of 1:800 (under 40x lens)
Immunofluorescent analysis of Hela cells, using DDX17 antibody Catalog No:109781 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DDX17,P72 (IP:Catalog No:109781, 4ug; Detection:Catalog No:109781 1:1000) with mouse brain tissue lysate 4000ug.
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Background
DDX17 represents DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
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References
- Koo CX, Kobiyama K, Shen YJ. RNA polymerase III regulates cytosolic RNA:DNA hybrids and intracellular microRNA expression. The Journal of biological chemistry. 290(12):7463-73. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"