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Product Name
DDX1 antibody
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Description
DDX1 Rabbit Polyclonal antibody. Positive IP detected in PC-3 cells. Positive WB detected in HeLa cells, COLO 320 cells, HEK-293 cells, PC-3 cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 82 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DBP RB antibody; DDX1 antibody; DEAD box protein 1 antibody; UKVH5d antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDX1 recombinant protein (Accession Number: NM_004939). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109779(DDX1 antibody) at dilution of 1:500
IP Result of anti-DDX1 (IP:Catalog No:109779, 3ug; Detection:Catalog No:109779 1:1000) with PC-3 cells lysate 5000ug.
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109779(DDX1 antibody) at dilution of 1:50 (under 10x lens)
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Background
DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.
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References
- Edgcomb SP, Carmel AB, Naji S. DDX1 is an RNA-dependent ATPase involved in HIV-1 Rev function and virus replication. Journal of molecular biology. 415(1):61-74. 2012.
- Chakrabarti A, Banerjee S, Franchi L. RNase L activates the NLRP3 inflammasome during viral infections. Cell host & microbe. 17(4):466-77. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"