DDHD2 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive WB detected in rat brain tissue, mouse lung tissue, mouse testis tissue. Observed molecular weight by Western-blot: 80-90 kDa
ELISA, WB, IF
Human, Mouse, Rat; other species not tested.
DDHD domain containing 2 antibody; DDHD2 antibody; KIAA0725 antibody; KIAA0725p antibody; Phospholipase DDHD2 antibody; SAMWD1 antibody
This antibody was obtained by immunization of DDHD2 recombinant protein (Accession Number: BC010504). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109772(DDHD2 Antibody) at dilution of 1:2000
Immunofluorescent analysis of HEK-293 cells using Catalog No:109772(DDHD2 Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa (PMID: 11788596).
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