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Product Name
DDB1 antibody
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Description
DDB1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in mouse testis tissue, HeLa cells, human brain tissue, human kidney tissue, human placenta tissue, Jurkat cells, rat testis tissue. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 127 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DDB p127 subunit antibody; DDB1 antibody; DDBA antibody; DNA damage binding protein 1 antibody; DNA damage binding protein a antibody; HBV X assOCiated protein 1 antibody; UV damaged DNA binding factor antibody; UV DDB 1 antibody; UV DDB1 antibody; XAP 1 antibody; XAP1 antibody; XPCE antibody; XPE antibody; XPE BF antibody; XPE binding factor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDB1 recombinant protein (Accession Number: NM_001923). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:109770(DDB1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109770(DDB1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-DDB1 (IP:Catalog No:109770, 5ug; Detection:Catalog No:109770 1:500) with Jurkat cells lysate 3080ug.
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Background
DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.
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References
- Kang X, Chen X, He Y. DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus replication. Virology. 435(2):385-94. 2013.
- Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X. 14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage. PloS one. 8(3):e55268. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"