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Product Name
DDAH1 Polyclonal Antibody
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Description
Polyclonal antibody to DDAH1
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
DDAH1 antibody; DDAH antibody; DDAH-1 antibody; DDAHI antibody; HEL-S-16 antibody; dimethylarginine dimethylaminohydrolase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH1 (NP_036269.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200 -
Validations
Western blot - DDAH1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DDAH1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"