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Product Name
DCTD antibody
- Documents
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Description
DCTD Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in human brain tissue, PC-3 cells. Observed molecular weight by Western-blot: 20kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dCMP deaminase antibody; DCTD antibody; Deoxycytidylate deaminase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DCTD recombinant protein (Accession Number: XM_017007820). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109758(DCTD antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using DCTD antibody Catalog No:109758 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).
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