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DCT antibody

Cat.#: 109757

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Product Information

  • Product Name
    DCT antibody
  • Documents
  • Description
    DCT Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF7 cells, rat skin tissue. Positive IP detected in HL-60 cells. Positive IHC detected in human skin tissue. Observed molecular weight by Western-blot: 59 kDa
  • Tested applications
    ELISA, IHC, WB, IP
  • Species reactivity
    Human,Mouse,Rat, Monkey; other species not tested.
  • Alternative names
    DCT antibody; L dopachrome Delta isomerase antibody; L dopachrome tautomerase antibody; TRP 2 antibody; TRP2 antibody; Tyrosinase related protein 2 antibody; TYRP2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DCT recombinant protein (Accession Number: NM_001922). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109757(DCT antibody) at dilution of 1:1000

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109757(DCT antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human skin using Catalog No:109757(DCT antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skin using Catalog No:109757(DCT antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skin using Catalog No:109757(DCT antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human skin using Catalog No:109757(DCT antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-DCT (IP:Catalog No:109757, 4ug; Detection:Catalog No:109757 1:500) with HL-60 cells lysate 4000ug.

    IP Result of anti-DCT (IP:Catalog No:109757, 4ug; Detection:Catalog No:109757 1:500) with HL-60 cells lysate 4000ug.

  • Background
    DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).
  • References
    • Strunnikova NV, Maminishkis A, Barb JJ. Transcriptome analysis and molecular signature of human retinal pigment epithelium. Human molecular genetics. 19(12):2468-86. 2010.
    • Luo C, Merz PR, Chen Y. MiR-101 inhibits melanoma cell invasion and proliferation by targeting MITF and EZH2. Cancer letters. 341(2):240-7. 2013.
    • Su TR, Lin JJ, Tsai CC. Inhibition of melanogenesis by gallic acid: possible involvement of the PI3K/Akt, MEK/ERK and Wnt/β-catenin signaling pathways in B16F10 cells. International journal of molecular sciences. 14(10):20443-58. 2013.
    • Luo C, Tetteh PW, Merz PR. miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes. The Journal of investigative dermatology. 133(3):768-75. 2013.
    • Igoucheva O, Grazzini M, Pidich A. Immunotargeting and eradication of orthotopic melanoma using a chemokine-enhanced DNA vaccine. Gene therapy. 20(9):939-48. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"