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Product Name
DCLRE1B antibody
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Description
DCLRE1B Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
5 exonuclease Apollo antibody; APOLLO antibody; DCLRE1B antibody; DKFZp686C24187 antibody; FLJ12810 antibody; FLJ13998 antibody; hSNM1B antibody; SNM1 homolog B antibody; SNM1B antibody; SNMIB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DCLRE1B recombinant protein (Accession Number: NM_022836). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109752(DCLRE1B antibody) at dilution of 1:800
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:109752(DCLRE1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Interstrand cross-links (ICLs) is an extremely cytotoxic form of DNA damage that covalently tethers the two strands of the double helix, which inhibits fundamental nuclear functions, including transcription and DNA replication [PMID:21701511]. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links [PMID:10848582]. Furthermore, DCLRE1B was identified as a TRF2-interacting protein and, has a role in telomere maintenance in association with the Shelterin complex, preventing non-homologous end-joining-mediated chromosome fusions protecting leading strand telomeres [PMID:20619712].
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