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Product Name
DCK antibody
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Description
DCK Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, Jurkat cells. Observed molecular weight by Western-blot: 30kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DCK antibody; deoxycytidine kinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DCK recombinant protein (Accession Number: NM_000788). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109750(DCK antibody) at dilution of 1:1000
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Background
Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443).
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References
- Rathe SK, Moriarity BS, Stoltenberg CB. Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia. Scientific reports. 4:6048. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"