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Product Name
DCI antibody
- Documents
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Description
DCI Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, BxPC-3 cells, human brain tissue, human kidney tissue, human lung tissue. Positive IF detected in BxPC-3 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 31kd
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
D2 enoyl CoA isomerase antibody; DCI antibody; Dodecenoyl CoA isomerase antibody; ECI1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DCI recombinant protein (Accession Number: NM_001178029). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109749(DCI antibody) at dilution of 1:50 (under 10x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109749(DCI Antibody) at dilution of 1:600
Immunofluorescent analysis of BxPC-3 cells using Catalog No:109749(DCI Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).
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