DCI antibody

Cat.#: 109749

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Product Information

  • Product Name
    DCI antibody
  • Documents
  • Description
    DCI Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, BxPC-3 cells, human brain tissue, human kidney tissue, human lung tissue. Positive IF detected in BxPC-3 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 31kd
  • Tested applications
    ELISA, IHC, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    D2 enoyl CoA isomerase antibody; DCI antibody; Dodecenoyl CoA isomerase antibody; ECI1 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DCI recombinant protein (Accession Number: NM_001178029). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109749(DCI antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109749(DCI antibody) at dilution of 1:50 (under 10x lens)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109749(DCI Antibody) at dilution of 1:600

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109749(DCI Antibody) at dilution of 1:600

    Immunofluorescent analysis of BxPC-3 cells using Catalog No:109749(DCI Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of BxPC-3 cells using Catalog No:109749(DCI Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

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