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Product Name
DCAF7 Polyclonal Antibody
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Description
Polyclonal antibody to DCAF7
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
DCAF7 antibody; AN11 antibody; HAN11 antibody; SWAN-1 antibody; WDR68 antibody; DDB1 and CUL4 associated factor 7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human DCAF7 (NP_005819.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - DCAF7 Polyclonal Antibody
Western blot analysis of extracts of 293T cells, using DCAF7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - DCAF7 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using DCAF7 antibody . Blue: DAPI for nuclear staining.
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Background
Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"