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Product Name
DAT antibody
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Description
DAT Rabbit Polyclonal antibody. Positive WB detected in rat brain tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in mouse brain tissue. Observed molecular weight by Western-blot: 68 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
DA transporter antibody; DAT antibody; DAT1 antibody; SLC6A3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DAT recombinant protein (Accession Number: NM_001044). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109869(SLC6A3 Antibody) at dilution of 1:600
IP Result of anti-SLC6A3 (IP:Catalog No:109869, 4ug; Detection:Catalog No:109869 1:600) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:109869(SLC6A3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:109869(SLC6A3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
SLC6A3 represents solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
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References
- Gou H, Wen D, Ma C. Protective effects of cholecystokinin-8 on methamphetamine-induced behavioral changes and dopaminergic neurodegeneration in mice. Behavioural brain research. 283:87-96. 2015.
- Sconce MD, Churchill MJ, Greene RE, Meshul CK. Intervention with exercise restores motor deficits but not nigrostriatal loss in a progressive MPTP mouse model of Parkinson's disease. Neuroscience. 299:156-74. 2015.
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