DARS2 antibody

DARS2 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

DARS2 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human brain tissue, human testis tissue. Positive WB detected in K-562 cells, human placenta tissue, U-937 cells. Observed molecular weight by Western-blot: 68-74 kDa, 50 kDa

ELISA, WB, IHC

Human,Mouse,Rat; other species not tested.

Aspartate tRNA ligase antibody; ASPRS antibody; DARS2 antibody; FLJ10514 antibody; LBSL antibody; MT ASPRS antibody; RP3 383J4.2 antibody

Rabbit IgG

This antibody was obtained by immunization of DARS2 recombinant protein (Accession Number: NM_018122). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).

• Dogan SA, Pujol C, Maiti P. Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Cell metabolism. 19(3):458-69. 2014.