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Product Name
DAPK1 antibody
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Description
DAPK1 Rabbit Polyclonal antibody. Positive WB detected in A549 cells. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 140-160 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DAP kinase 1 antibody; DAPK antibody; DAPK1 antibody; DKFZp781I035 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DAPK1 recombinant protein (Accession Number: NM_001288730). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:109862(DAPK1 Antibody) at dilution of 1:600
Immunofluorescent analysis of A549 cells using Catalog No:109862(DAPK1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784). This protein has 3 isoforms produced by alternative splicing.
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