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Product Name
DAD1 antibody
- Documents
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Description
DAD1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells. Observed molecular weight by Western-blot: 16-20 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DAD 1 antibody; DAD1 antibody; defender against cell death 1 antibody; OST2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DAD1 recombinant protein (Accession Number: NM_001344). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109855(DAD1 Antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:109855(DAD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DAD1 (defender against cell death 1) is a downstream target of the NFkB survival pathway and exhibits an antiapoptotic function. It is also named as OST2 (Oligosaccharyl transferase2) and catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Increased expression of DAD1 has been observed in various tumors.
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