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Product Name
DAAM1 antibody
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Description
DAAM1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Positive IP detected in mouse liver tissue. Positive WB detected in human heart tissue, HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, mouse testis tissue. Observed molecular weight by Western-blot: 123kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DAAM1 antibody; FLJ41657 antibody; KIAA0666 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DAAM1 recombinant protein (Accession Number: NM_001270520). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109818(DAAM1 antibody) at dilution of 1:500
IP Result of anti-DAAM1 (IP:Catalog No:109818, 3ug; Detection:Catalog No:109818 1:500) with mouse liver tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human brain using Catalog No:109818(DAAM1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109818(DAAM1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using DAAM1 antibody Catalog No:109818 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.
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References
- Li D, Hallett MA, Zhu W. Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis. Development (Cambridge, England). 138(2):303-15. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"