Cytokeratin 6A-specific antibody

Cat.#: 107010

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Product Information

  • Product Name
    Cytokeratin 6A-specific antibody
  • Documents
  • Description
    Cytokeratin 6A-specific Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HeLa cells, mouse skin tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 56 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CK 6A antibody; CK 6D antibody; CK6A antibody; CK6C antibody; CK6D antibody; Cytokeratin 6A antibody; Cytokeratin 6D antibody; Hom s 5 antibody; K6A antibody; K6C antibody; K6D antibody; keratin 6A antibody; KRT conserve antibody; KRT6 antibody; KRT6A antibody; KRT6C antibody; KRT6D antibody; Type II keratin Kb6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_005554). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

  • Validations

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109808(KRT6-specific antibody) at dilution of 1:300

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109808(KRT6-specific antibody) at dilution of 1:300

    IP Result of anti-KRT6-specific (IP:Catalog No:109808, 4ug; Detection:Catalog No:109808 1:300) with HeLa cells lysate 520ug.

    IP Result of anti-KRT6-specific (IP:Catalog No:109808, 4ug; Detection:Catalog No:109808 1:300) with HeLa cells lysate 520ug.

  • Background
    Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"