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Product Name
Cytokeratin 6A-specific antibody
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Description
Cytokeratin 6A-specific Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HeLa cells, mouse skin tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CK 6A antibody; CK 6D antibody; CK6A antibody; CK6C antibody; CK6D antibody; Cytokeratin 6A antibody; Cytokeratin 6D antibody; Hom s 5 antibody; K6A antibody; K6C antibody; K6D antibody; keratin 6A antibody; KRT conserve antibody; KRT6 antibody; KRT6A antibody; KRT6C antibody; KRT6D antibody; Type II keratin Kb6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005554). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109808(KRT6-specific antibody) at dilution of 1:300
IP Result of anti-KRT6-specific (IP:Catalog No:109808, 4ug; Detection:Catalog No:109808 1:300) with HeLa cells lysate 520ug.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.
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