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Product Name
Cytokeratin 6A antibody
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Description
Cytokeratin 6A Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in A431 cells, Hela cells. Positive IHC detected in human lung cancer tissue, human oesophagus tissue, human prostate cancer tissue, human skin tissue, human tonsil tissue. Positive WB detected in A431 cells, A375 cells, HeLa cells, MCF7 cells, rat skin tissue. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, WB, FC, IF, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CK 6A antibody; CK 6D antibody; CK6A antibody; CK6C antibody; CK6D antibody; Cytokeratin 6A antibody; Cytokeratin 6D antibody; Hom s 5 antibody; K6A antibody; K6C antibody; K6D antibody; keratin 6A antibody; KRT conserve antibody; KRT6 antibody; KRT6A antibody; KRT6C antibody; KRT6D antibody; Type II keratin Kb6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cytokeratin 6A recombinant protein (Accession Number: NM_005554). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109807(KRT6A antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:109807(KRT6A Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:109807(KRT6A Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of A431 cells using Catalog No:109807(KRT6A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug KRT6A antibody (Catalog No:109807, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.
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