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Product Name
Cytokeratin 15 antibody
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Description
Cytokeratin 15 Mouse Monoclonal antibody. Positive IHC detected in human oesophagus cancer tissue, human skin cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in A431 cells. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
CK 15 antibody; CK15 antibody; Cytokeratin 15 antibody; K15 antibody; K1CO antibody; keratin 15 antibody; KRT15 antibody; KRTB antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Cytokeratin 15 recombinant protein (Accession Number: NM_002275). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:100-1:1000
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:107230(KRT15 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:107230(KRT15 Antibody) at dilution of 1:500 (under 10x lens).
Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:107230(KRT15 Antibody) at dilution of 1:500 (under 40x lens).
Immunofluorescent analysis of HeLa cells using Catalog No:107230(KRT15 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
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