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Product Name
Cystatin C antibody
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Description
Cystatin C Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue, human brain tissue. Positive IF detected in HeLa cells. Positive WB detected in human brain tissue, fetal human brain tissue. Observed molecular weight by Western-blot: 16 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARMD11 antibody; CST3 antibody; Cystatin 3 antibody; cystatin C antibody; CYTC antibody; Gamma trace antibody; Post gamma globulin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cystatin C recombinant protein (Accession Number: NM_000099). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109715(CST3 antibody) at dilution of 1:400
Immunohistochemistry of paraffin-embedded human ovary tissue slide using Catalog No:109715(Cystatin C Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human ovary tissue slide using Catalog No:109715(Cystatin C Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109715(Cystatin C Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CST3 represents cystatin C
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References
- Collins MA, An J, Peller D, Bowser R. Total protein is an effective loading control for cerebrospinal fluid western blots. Journal of neuroscience methods. 251:72-82. 2015.
- Yan Y, Zhou K, Wang L, Zhou Y, Chen X, Fan Q. Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma. International journal of clinical and experimental pathology. 8(9):10102-11. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"