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Product Name
CYPC antibody
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Description
CYPC Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, mouse kidney tissue. Positive WB detected in HEK-293 cells, human heart tissue, mouse heart tissue. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cyclophilin C antibody; CYPC antibody; PPIase C antibody; PPIC antibody; Rotamase C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYPC recombinant protein (Accession Number: NM_000943). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109713(CYPC antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:109713(CYPC Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:109713(CYPC Antibody) at dilution of 1:50 (under 40x lens)
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Background
Cyclophilin C also named as peptidyl-prolyl cis-trans isomerase C(PPIC),catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. It is expressed most highly in the kidney and can be detected in activated T cells(PMID:1652374).
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References
- Chatterji U, Bobardt M, Selvarajah S. The isomerase active site of cyclophilin A is critical for hepatitis C virus replication. The Journal of biological chemistry. 284(25):16998-7005. 2009.
- Stocki P, Chapman DC, Beach LA, Williams DB. Depletion of cyclophilins B and C leads to dysregulation of endoplasmic reticulum redox homeostasis. The Journal of biological chemistry. 289(33):23086-96. 2014.
- Chapman DC, Stocki P, Williams DB. Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules. PloS one. 10(12):e0145458. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"