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Product Name
CYP7B1 antibody
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Description
CYP7B1 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HEK-293 cells, mouse liver tissue. Positive IHC detected in human brain tissue, human small intestine tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CBAS3 antibody; CP7B antibody; CYP7B1 antibody; CytOChrome P450 7B1 antibody; Oxysterol 7 alpha hydroxylase antibody; SPG5A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYP7B1 recombinant protein (Accession Number: NM_004820). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109712(CYP7B1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:109712(CYP7B1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:109712(CYP7B1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:109712(CYP7B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"