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Product Name
CYP7A1 Polyclonal Antibody
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Description
Polyclonal antibody to CYP7A1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
CYP7A1 antibody; CP7A antibody; CYP7 antibody; CYPVII antibody; cholesterol 7-alpha-monooxygenase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human CYP7A1 (NP_000771.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CYP7A1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CYP7A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.
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Background
Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"