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Product Name
CYP3A7 Polyclonal Antibody
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Description
Polyclonal antibody to CYP3A7
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CYP3A7 antibody; CP37 antibody; CYPIIIA7 antibody; P-450 antibody; P-450111A7 antibody; P450-HFLA antibody; cytochrome P450 3A7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A7 (NP_000756.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CYP3A7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CYP3A7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - CYP3A7 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CYP3A7 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"