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Product Name
CYP2C9 Polyclonal Antibody
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Description
Polyclonal antibody to CYP2C9
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
CYP2C9 antibody; CPC9 antibody; CYP2C antibody; CYP2C10 antibody; CYPIIC9 antibody; P450IIC9 antibody; cytochrome P450 2C9 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 211-490 of human CYP2C9 (NP_000762.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - CYP2C9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CYP2C9 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 10s.
Immunohistochemistry - CYP2C9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using CYP2C9 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - CYP2C9 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CYP2C9 antibody . Blue: DAPI for nuclear staining.
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Background
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"