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Product Name
CYP2C18 Polyclonal Antibody
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Description
Polyclonal antibody to CYP2C18
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CYP2C18 antibody; CPCI antibody; CYP2C antibody; CYP2C17 antibody; P450-6B/29C antibody; P450IIC17 antibody; cytochrome P450 2C18 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 231-490 of human CYP2C18 (NP_000763.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CYP2C18 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CYP2C18 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CYP2C18 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using CYP2C18 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CYP2C18 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using CYP2C18 antibody at dilution of 1:100 (40x lens).
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Background
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"