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Product Name
CYP27B1 Polyclonal Antibody
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Description
Polyclonal antibody to CYP27B1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CYP27B1 antibody; CP2B antibody; CYP1 antibody; CYP1alpha antibody; CYP27B antibody; P450c1 antibody; PDDR antibody; VDD1 antibody; VDDR antibody; VDDRI antibody; VDR antibody; cytochrome P450 family 27 subfamily B member 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-195 of human CYP27B1 (NP_000776.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:500
IF 1:20 - 1:50 -
Validations
Western blot - CYP27B1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CYP27B1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - CYP27B1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CYP27B1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"