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Product Name
CYP27A1 antibody
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Description
CYP27A1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive WB detected in mouse liver tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 60-65kd
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CP27 antibody; CTX antibody; CYP27 antibody; CYP27A1 antibody; CytOChrome P 450C27/25 antibody; CytOChrome P450 27 antibody; Sterol 27 hydroxylase antibody; Vitamin D(3) 25 hydroxylase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYP27A1 recombinant protein (Accession Number: NM_000784). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human liver using Catalog No:109690(CYP27A1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:109690(CYP27A1 antibody) at dilution of 1:50 (under 40x lens)
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109690(CYP27A1 Antibody) at dilution of 1:600
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Background
Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.
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References
- Dasari B, Prasanthi JR, Marwarha G, Singh BB, Ghribi O. Cholesterol-enriched diet causes age-related macular degeneration-like pathology in rabbit retina. BMC ophthalmology. 11:22. 2011.
- Jia X, Naito H, Yetti H. Dysregulated bile acid synthesis, metabolism and excretion in a high fat-cholesterol diet-induced fibrotic steatohepatitis in rats. Digestive diseases and sciences. 58(8):2212-22. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"