Product Name

CYP1B1 antibody

Documents

Description

CYP1B1 Rabbit Polyclonal antibody. Positive WB detected in MCF-7 cells, mouse heart tissue, PC-3 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 52 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

CP1B antibody; CYP1B1 antibody; CYPIB1 antibody; CytOChrome P450 1B1 antibody; GLC3A antibody; P4501B1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of CYP1B1 recombinant protein (Accession Number: NM_000104). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

Validations

MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109685(CYP1B1 Antibody) at dilution of 1:600

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:109685(CYP1B1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:109685(CYP1B1 Antibody) at dilution of 1:50 (under 40x lens)

Background

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

CYP1B1 antibody

Product Information

Related Products / Services