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Product Name
CYP11B2 Polyclonal Antibody
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Description
Polyclonal antibody to CYP11B2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CYP11B2 antibody; ALDOS antibody; CPN2 antibody; CYP11B antibody; CYP11BL antibody; CYPXIB2 antibody; P-450C18 antibody; P450C18 antibody; P450aldo antibody; cytochrome P450 11B2, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B2 (NP_000489.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - CYP11B2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CYP11B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 20s.
Immunohistochemistry - CYP11B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat adrenal gland using CYP11B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CYP11B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse adrenal gland using CYP11B2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - CYP11B2 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CYP11B2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"