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Cyclin B1 antibody

Cat.#: 109662

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Product Information

  • Product Name
    Cyclin B1 antibody
  • Documents
  • Description
    Cyclin B1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, PC-3 cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 55-60 kDa
  • Tested applications
    ELISA, WB, IP, IF, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CCNB antibody; CCNB1 antibody; cyclin B1 antibody; G2/mitotic specific cyclin B1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_031966). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109662(CCNB1 antibody) at dilution of 1:500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109662(CCNB1 antibody) at dilution of 1:500

    IP Result of anti-Cyclin B1 (IP:Catalog No:109662, 4ug; Detection:Catalog No:109662 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-Cyclin B1 (IP:Catalog No:109662, 4ug; Detection:Catalog No:109662 1:500) with HeLa cells lysate 1200ug.

    Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:109662(Cyclin B1 Antibody) at dilution of 1:50 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:109662(Cyclin B1 Antibody) at dilution of 1:50 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:109662(Cyclin B1 Antibody) at dilution of 1:50 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:109662(Cyclin B1 Antibody) at dilution of 1:50 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunofluorescent analysis of HeLa cells using Catalog No:109662(Cyclin B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:109662(Cyclin B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1.
  • References
    • Zheng L, Wang X, Luo W, Zhan Y, Zhang Y. Brucine, an effective natural compound derived from nux-vomica, induces G1 phase arrest and apoptosis in LoVo cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 58:332-9. 2013.
    • Meng X, Wang X, Tian X, Yang Z, Li M, Zhang C. Protection of neurons from high glucose-induced injury by deletion of MAD2B. Journal of cellular and molecular medicine. 18(5):844-51. 2014.
    • Chen H, Xu L, Yin L. iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells. Proteomics. 14(1):51-73. 2014.
    • Liu Z, Yang L, Teng X, Zhang H, Guan H. The involvement of CXCR7 in modulating the progression of papillary thyroid carcinoma. The Journal of surgical research. 191(2):379-88. 2014.
    • Dai B, Zhan Y, Qi J, Zhang Y. Eupolyphaga sinensis Walker inhibits human chronic myeloid leukemia cell K562 growth by inducing G2-M phase cell cycle arrest and targeting EGFR signaling pathway and in S180 tumor-bearing mice. Environmental toxicology and pharmacology. 37(3):1177-85. 2014.
    • Zhang Y, Zhan Y, Zhang D. Eupolyphaga sinensis walker displays inhibition on hepatocellular carcinoma through regulating cell growth and metastasis signaling. Scientific reports. 4:5518. 2014.
    • Li X, Liu X, Xu Y. KLF5 promotes hypoxia-induced survival and inhibits apoptosis in non-small cell lung cancer cells via HIF-1α. International journal of oncology. 45(4):1507-14. 2014.
    • Chen X, Meng Q, Wang C. Protective effects of calycosin against CCl4-induced liver injury with activation of FXR and STAT3 in mice. Pharmaceutical research. 32(2):538-48. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"