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Product Name
CYB5R3 antibody
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Description
CYB5R3 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, A431 cells, HeLa cells, human liver tissue, human lung tissue, human placenta tissue, MCF7 cells. Positive IP detected in HepG2 cells. Positive IHC detected in human lung cancer tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 30-35 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B5R antibody; CYB5R3 antibody; CytOChrome b5 reductase antibody; cytOChrome b5 reductase 3 antibody; DIA1 antibody; Diaphorase 1 antibody; NADH cytOChrome b5 reductase 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYB5R3 recombinant protein (Accession Number: NM_000398). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109657(CYB5R3 antibody) at dilution of 1:1000
IP Result of anti-CYB5R3 (IP:Catalog No:109657, 3ug; Detection:Catalog No:109657 1:1000) with HepG2 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109657(CYB5R3 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CYB5R3 antibody Catalog No:109657 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.
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References
- Rahaman MM, Reinders FG, Koes D. Structure Guided Chemical Modifications of Propylthiouracil Reveal Novel Small Molecule Inhibitors of Cytochrome b5 Reductase 3 That Increase Nitric Oxide Bioavailability. The Journal of biological chemistry. 290(27):16861-72. 2015.
- Samhan-Arias AK, Garcia-Bereguiain MA, Martin-Romero FJ, Gutierrez-Merino C. Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane. Molecular and cellular neurosciences. 40(1):14-26. 2009.
- Ramirez DM, Andersson S, Russell DW. Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain. The Journal of comparative neurology. 507(5):1676-93. 2008.
- Marques-da-Silva D, Samhan-Arias AK, Tiago T, Gutierrez-Merino C. L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane. Journal of proteomics. 73(8):1502-10. 2010.
- Samhan-Arias AK, Marques-da-Silva D, Yanamala N, Gutierrez-Merino C. Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons. Journal of proteomics. 75(10):2934-49. 2012.
- Samhan-Arias AK, Gutierrez-Merino C. Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite. Free radical biology & medicine. 73:174-89. 2014.
- Marques-da-Silva D, Gutierrez-Merino C. Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling. Cell calcium. 56(2):108-23. 2014.
- Samhan-Arias AK, López-Sánchez C, Marques-da-Silva D. High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain. Brain structure & function. 2015.
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