CX3CR1 antibody

Cat.#: 109634

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Product Information

  • Product Name
    CX3CR1 antibody
  • Documents
  • Description
    CX3CR1 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive FC detected in K-562 cells. Positive IP detected in SH-SY5Y cells. Positive WB detected in HL-60 cells, K-562 cells, SH-SY5Y cells, U-937 cells. Observed molecular weight by Western-blot: 47 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Beta chemokine receptor like 1 antibody; C X3 C CKR 1 antibody; CCRL1 antibody; CMK BRL 1 antibody; CMK BRL1 antibody; CMKBRL1 antibody; CMKDR1 antibody; CX3C chemokine receptor 1 antibody; CX3CR1 antibody; Fractalkine receptor antibody; G protein coupled receptor 13 antibody; GPR13 antibody; GPRV28 antibody; V28 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CX3CR1 recombinant protein (Accession Number: NM_001337). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109634(CX3CR1 antibody) at dilution of 1:800

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109634(CX3CR1 antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109634(CX3CR1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109634(CX3CR1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109634(CX3CR1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109634(CX3CR1 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-CX3CR1 (IP:Catalog No:109634, 4ug; Detection:Catalog No:109634 1:1000) with SH-SY5Y cells lysate 1600ug.

    IP Result of anti-CX3CR1 (IP:Catalog No:109634, 4ug; Detection:Catalog No:109634 1:1000) with SH-SY5Y cells lysate 1600ug.

    1X10^6 K-562 cells were stained with 0.2ug CX3CR1 antibody (Catalog No:109634, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 K-562 cells were stained with 0.2ug CX3CR1 antibody (Catalog No:109634, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).
  • References
    • Yang X, Yan J, Feng J. Treatment with tanshinone IIA suppresses disruption of the blood-brain barrier and reduces expression of adhesion molecules and chemokines in experimental autoimmune encephalomyelitis. European journal of pharmacology. 771:18-28. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"