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Product Name
CWC15 antibody
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Description
CWC15 Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue, human breast cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in mouse liver tissue, HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, PC-3 cells, SW 1990 cells. Observed molecular weight by Western-blot: 27-35 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C11orf5 antibody; CWC15 antibody; Cwf15 antibody; HSPC148 antibody; ORF5 antibody; Protein CWC15 homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CWC15 recombinant protein (Accession Number: NM_016403). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109630(CWC15 Antibody) at dilution of 1:600
IP Result of anti-CWC15 (IP:Catalog No:109630, 3ug; Detection:Catalog No:109630 1:600) with HeLa cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:109630(CWC15 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:109630(CWC15 Antibody) at dilution of 1:50 (under 40x lens)
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Background
CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
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References
- van Maldegem F, Maslen S, Johnson CM. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic acids research. 43(14):7058-69. 2015.
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