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Product Name
CUL4B antibody
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Description
CUL4B Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HEK-293 cells, HeLa cells, human testis tissue, mouse testis tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 102 kDa
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CUL 4B antibody; CUL4B antibody; cullin 4B antibody; DKFZp686F1470 antibody; KIAA0695 antibody; MRXHF2 antibody; MRXSC antibody; SFM2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUL4B recombinant protein (Accession Number: NM_003588). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109746(CUL4B antibody) at dilution of 1:1000
IP Result of anti-CUL4B (IP:Catalog No:109746, 3ug; Detection:Catalog No:109746 1:1000) with HeLa cells lysate 4650ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109746(CUL4B antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CUL4B antibody Catalog No:109746 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).
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References
- Liu L, Yin Y, Li Y. Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis. Cell research. 22(8):1258-69. 2012.
- Yoshizawa T, Karim MF, Sato Y. SIRT7 controls hepatic lipid metabolism by regulating the ubiquitin-proteasome pathway. Cell metabolism. 19(4):712-21. 2014.
- Chen YA, Peng YJ, Hu MC. The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels. Scientific reports. 5:10667. 2015.
- Antonioli M, Albiero F, Nazio F. AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics. Developmental cell. 31(6):734-46. 2014.
- Yin Y, Liu L, Yang C. Cell autonomous and non-autonomous function of CUL4B in Mouse Spermatogenesis. The Journal of biological chemistry. 2016.
- Yamashita K, Ide M, Furukawa KT, Suzuki A, Hirano H, Ohno S. Tumor suppressor protein Lgl mediates G1 cell cycle arrest at high cell density by forming an Lgl-VprBP-DDB1 complex. Molecular biology of the cell. 26(13):2426-38. 2015.
- Liu HC, Enikolopov G, Chen Y. Cul4B regulates neural progenitor cell growth. BMC neuroscience. 13:112. 2012.
- Zhang S, Zhao H, Darzynkiewicz Z. A novel function of CRL4(Cdt2): regulation of the subunit structure of DNA polymerase δ in response to DNA damage and during the S phase. The Journal of biological chemistry. 288(41):29550-61. 2013.
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