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Product Name
CUL3 antibody
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Description
CUL3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IHC detected in human prostate cancer tissue, human heart tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 89kd
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CUL 3 antibody; CUL3 antibody; cullin 3 antibody; KIAA0617 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUL3 recombinant protein (Accession Number: NM_003590). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109743(CUL3 Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109743(CUL3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:109743(CUL3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:109743(CUL3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.
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