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Product Name
CUL2 antibody
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Description
CUL2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human prostate cancer tissue, human kidney tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 87 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CUL 2 antibody; CUL2 antibody; cullin 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUL2 recombinant protein (Accession Number: NM_003591). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109742(CUL2 antibody) at dilution of 1:100
IP Result of anti-CUL2 (IP:Catalog No:109742, 4ug; Detection:Catalog No:109742 1:200) with HeLa cells lysate 2500ug.
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109742(CUL2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CUL2 antibody Catalog No:109742 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1–3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
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References
- Chen H, Ma H, Inuzuka H. DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase. Molecular and cellular biology. 33(6):1139-48. 2013.
- Antonioli M, Albiero F, Nazio F. AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics. Developmental cell. 31(6):734-46. 2014.
- Su Y, Ni Z, Wang G. Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p. International immunopharmacology. 13(4):468-75. 2012.
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