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Product Name
CTSH Polyclonal Antibody
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Description
Polyclonal antibody to CTSH
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
CTSH antibody; ACC-4 antibody; ACC-5 antibody; ACC4 antibody; ACC5 antibody; CPSB antibody; cathepsin H antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-335 of human CTSH (NP_004381.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CTSH Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CTSH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Important for the overall degradation of proteins in lysosomes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"